Agustina Sus Andreani, Anastasya Curie Sitanggang, Arvita Wulan Purnamasari, F. Widhi Mahatmanti, Mohammad Alauhdin, Muhammad Eka Prastya
Synthesis of gold nanoparticles (AuNPs) has been carried out with α-cyclodextrin (αCDs) and β-cyclodextrin (βCDs) as reducing and stabilizing agents to have antibacterial properties. Redwine-colored solutions resulted from the synthesis of AuNPs-αCDs and AuNPs-βCDs, which showed absorbance from SPR 518 nm at UV-Vis of 0.526 and 0.540, respectively. The spherical shape is the result from the Transmission Electron Microscope (TEM) of AuNPs-αCDs is 8.31 ± 0.09 nm and AuNPs-βCDs is 12.23 ± 0.31 nm. The antibacterial ability of AuNPs-αCDs and AuNPs-βCDs was compared with the Total Plate Count (TPC) method using a bacterial concentration in each sample of 5 × 103 CFU/mL, 5 × 102 CFU/mL, and 5 × 101 CFU/mL carried out using gram-positive bacteria (Bacillus Subtilis and Staphylococcus aureus) and gram-negative bacteria (Escherichia Coli and Pseudomonas Aeruginosa). TPC method was done with two repetitions when AuNPs-αCDs and AuNPs-βCDs showed good antibacterial activity on bacteria Staphylococcus aureus, which is supported by the result of the bar chart. TEM data was obtained Staphylococcus aureus bacteria have a round-shaped and when interacting with AuNPs-CDs there is a leak in the outer membrane of the Staphylococcus aureus bacteria. © 2026 American Institute of Physics Inc.. All rights reserved.
Research Centre for Chemistry, National Research and Innovation Agency (BRIN), Kawasan Puspiptek, Building 452, Serpong, Tangerang Selatan, Banten, 15314, Indonesia; Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Negeri Semarang, Central Java, Semarang, 50229, Indonesia; Research Centre for Pharmaceutical Ingredients and Traditional Medicine, National Research and Innovation Agency (BRIN), Kawasan Puspiptek Serpong, Tangerang Selatan, Banten, 15314, Indonesia
