Yuan Alfinsyah Sihombing, Uperianti, Rizky Indah Sari, Beni Rio Hermanto, Murni Handayani, Samuel Priyantoro Kusumocahyo, Mokhamad Fakhrul Ulum, Rikson Siburian, Cepi Kurniawan, Nuni Widiarti, Yeni Wahyuni Hartati, Isa Anshori
In the development of biosensors, it is essential to have sensors that provide rapid responses, exhibit high sensitivity and selectivity, and are non-invasive, such as screen-printed carbon electrode-based biosensors. In this study, SPCE-based and fabric-based biosensors were fabricated by modifying the working electrode (WE) surface using functionalized Multi-walled Carbon Nanotube/AgNi nanocomposites (f-MWCNT/AgNi) to enhance the biosensor's performance in detecting uric acid (UA). The successful synthesis of the f-MWCNT/AgNi nanomaterial was confirmed through UV–Vis, Raman, SEM–EDX, and XRD analyses. The f-MWCNT/AgNi nanomaterials were deposited on the WE surface using drop-casting. Subsequently, electrochemical characteristic tests and UA detection performance were conducted using cyclic voltammetry (CV) and differential pulse voltammetry (DPV) methods. The DPV curves revealed sensitivities of 27.699 μA/mM and 4.638 μA/mM for SPCE-based and fabric-based electrodes, respectively. The limit of detection (LOD) values for UA detection were 0.024 and 0.017 mM, with linearity (R2) 0.997 and 0.999 observed within the linear ranges of 0.05–1.00 and 1.0–5.0 mM, respectively. Both biosensors exhibited strong selectivity for UA against other components, including ascorbic acid, glucose, lactic acid, and ethanol. Based on these parameter values, f-MWCNT/AgNi-modified SPCE and fabric-based electrodes can be promoted as biosensors for uric acid detection. © 2024
Department of Physics, Faculty of Mathematics and Natural Sciences, Universitas Sumatera Utara, Jl. Bioteknologi No.1, Medan, 20155, Indonesia; Department of Physics, Faculty of Mathematics and Natural Sciences, Institut Teknologi Bandung, Jl. Ganesha No. 10, Bandung, 40132, Indonesia; Lab-on-Chip Group, Biomedical Engineering Department, School of Electrical Engineering and Informatics, Institut Teknologi Bandung, Bandung, 40132, Indonesia; Research Center for Nanotechnology Systems, National Research and Innovation Agency (BRIN), Puspiptek Area, Banten, Tangerang Selatan, 15314, Indonesia; Department of Chemical Engineering, Pamulang University (UNPAM), Pamulang, Banten, Tangerang Selatan, 15417, Indonesia; Department of Chemical Engineering, Faculty of Life Sciences and Technology, Swiss German University, Tangerang, 15143, Indonesia; School of Veterinary Medicine and Biomedical Sciences, IPB University (Bogor Agricultural University), Jalan Agathis Kampus IPB Dramaga, Bogor, 16680, Indonesia; Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Sumatera Utara, Medan, 20155, Indonesia; Chemistry Department, Faculty of Mathematics and Natural Sciences, Universitas Negeri Semarang, Semarang, 50229, Indonesia; Department of Chemistry, Faculty of Mathematics and Natural Science, Padjadjaran University, Jatinangor, 45363, Indonesia; Research Center for Nanosciences and Nanotechnology (RCNN), Bandung Institute of Technology, Bandung, 40132, Indonesia
