Yan Wisnu Prajoko, Agung Putra, Ardi Prasetio, Nur Dina Amalina
Aim To determine the effect of hypoxic mesenchymal stem cells (MSCs) on the interleukin (IL)-10 and interferon (IFN)-gamma in peripheral blood mononuclear cells (PBMCs) from systemic lupus erythematosus (SLE) patients. Methods This study used a post-test control group design. Hypoxic MSCs were isolated and characterized according to their surface marker expression and differentiation capacities. PBMCs isolated from SLE patients were divided into three groups: control and two treatment groups. The treatment groups were treated by co-cultu-ring MSCs to PBMCs with a ratio of 1:10 (T1) and 1:1 (T2) for 48 h incubation. Furthermore, IFN-gamma and IL-10 levels were determined by cytometric bead array (CBA) flow cytometry. Results Hypoxic MSCs significantly decreased the IFN-gamma levels and increased the IL-10 levels in dose-dependent manner compared to the control group. The highest activity of hypoxic MSCs was noticed in T2 group. Conclusion Hypoxic MSCs-induced IL-10 are important in the control of anti-inflammatory effect on SLE through inhibiting IFN-gamma. © 2024, Medical Association of Zenica-Doboj Canton. All rights reserved.
Department of Surgery, Faculty of Medicine, Universitas Diponegoro, Semarang, Indonesia; Stem Cell and Cancer Research, Semarang, Indonesia; Department of Postgraduate Biomedical Science, Faculty of Medicine, Universitas Islam Sultan Agung, Semarang, Indonesia; Department of Pathology Anatomy, Faculty of Medicine, Universitas Islam Sultan Agung, Semarang, Indonesia; Pharmaceutical Sciences Department, Faculty of Medicine, Universitas Negeri Semarang, Semarang, Indonesia
