Microencapsulation of Citrus Hystrix Essential Oil by Gelatin B/Chitosan Complex Coacervation Technique

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Siti Afiqah ‘Aisyah Murtadza, Nurul Asyikin Md Zaki, Junaidah Jai, Fazlena Hamzah, Nur Suhanawati Ashaari, Dewi Selvia Fardhyanti, Megawati, Nadya Alfa Cahaya Imani

2024 Pertanika Journal of Science and Technology Vol. 32 Issue 2 Article Cited by 1 Quartile

Abstract

Complex coacervation is an encapsulation technique used to preserve the bio functionality of essential oils as well as provide controlled release. In this present work, encapsulation of Citrus Hystrix essential oil (CHEO) was formed by a complex coacervation technique with Gelatin-B (Gel B) and Chitosan (Chi) as the capping materials. The suitable encapsulation formulation was investigated as a function of pH and wall ratio using Zeta Potential analysis. Turbidity measurement and coacervate yield were carried out to confirm the suitable condition. Total Phenolic Content (TPC) was used to obtain the encapsulation efficiency (EE%) of the process. Results show that the suitable condition for coacervate formation between Gel B and Chi ratio of 5:1 was at pH 5.8, which produced a high encapsulation efficiency of 94.81% ± 2.60. FTIR analysis validates the formation of coacervate as well as the encapsulated CHEO. The encapsulates obtained were spherical and dominated by 194.557 um particles. The CHEO was successfully encapsulated by a complex coacervation method. © Universiti Putra Malaysia Press.

Affiliations

School of Chemical Engineering, College of Engineering, Universiti Teknologi MARA, , Selangor, Shah Alam, 40450, Malaysia; Malaysia Genome and Vaccine Institute, National Institutes of Biotechnology Malaysia, Jalan Bangi, Selangor, Kajang, 43000, Malaysia; Department of Chemical Engineering, Faculty of Engineering, Universitas Negeri Semarang, Gunungpati, Semarang, 50229, Indonesia