Muchamad Dafip, Aprilia Indra Kartika, Meutia Srikandi Fitria, Winarto Reki, Muhammad Hussein Gasem, Retno Sri Iswari
Atherosclerosis is triggered by cholesterol accumulation in endothelial layers that induces the production of inflammatory cytokines, including TNF-α, IL-6, and IL-10. This condition disrupts microRNA homeostasis like miR-29b-3p, generally maintaining artery health. Steamed tomatoes contain higher antioxidant properties than raw, which might be better against atherosclerosis. However, its influence on inflammation and miR-29b-3p balance remains unclear. This study aims to elucidate the effect of steamed tomatoes on the TNF-α, IL-6, and IL-10 levels and miR-29b-3p expression under atherosclerosis conditions. Sprague Dawley male rats were equally divided into K1 group of healthy rats given a placebo; K2 group was atherosclerosis rats induced with 2 m of cholesterin/ 200 g/KgBW per day, then K3 and K4 groups were atherosclerosis rats supplemented with 20 mg/ 200 g/KgBW per day of atorvastatin and 16 mg/ 200 g/KgBW per day of steamed-tomato extract for 60 days. Steamed tomato decreases cytokine level in the K4 group and significantly differs from all groups (p < 0.050). This study showed that increased proinflammatory cytokine, including TNF-α and IL-6, is hypothetically involved in plaque formation and lamina layer destruction in endothelial. Steamed tomato supplementation also significantly increases miR-29b-3p expression to 0.98 ± 0.33 log10 fold change higher than K2 and K3 (p = 0.000). This study demonstrated the potential of steamed tomatoes to improve dyslipidemia, atherosclerosis-related inflammation, and miRNA homeostasis. This research provides additional knowledge regarding determining the expression of miR-29b-3p, which has the potential to be developed as a diagnostic or therapeutic target. © 2024, Universitas Negeri Semarang. All rights reserved.
Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Semarang, Indonesia; Magister Program of Biomedical Science, Faculty of Medicine, Universitas Diponegoro, Indonesia; Medical Laboratory Technology, Faculty of Nursing and Health Science, Universitas Muhammadiyah Semarang, Jl Kedungmundu Raya No 18, Indonesia; Department of Clinical Microbiology, Faculty of Medicine, Universitas Diponegoro, Indonesia; Center for Tropical and Infectious Disease (CENTRID), Dr Kariadi Hospital, Universitas Diponegoro, Indonesia
