Zuhriyan Ash Shiddieqy Bahlawan, Megawati, Ria Desiriani, Widi Astuti, Forita Dyah Arianti, Agung Kurnia Yahya, Hafiz Muneer Ahmad, Boram Yun, Andri Cahyo Kumoro, Deni Fajar Fitriyana, Achmad Yanuar Maulana, Jongsik Kim
Cow rumen waste (CRW), a slaughterhouse by-product rich in lignocellulose, can be directly converted into bioethanol without harsh chemical pretreatment or additional microbial inocula. In this study, we developed an integrated enzymatic hydrolysis and fermentation process to produce bioethanol from CRW using Viscozyme Cassava CL and Saccharomyces cerevisiae. Key process parameters for enzymatic hydrolysis (enzyme concentration, pH, and substrate loading) were optimized, with 3 wt% enzyme at pH 5.0 and 50 g L− 1 CRW yielding the highest glucose release. The enzymatic hydrolysate was then fermented by S. cerevisiae under optimal conditions (inoculum 3 wt%, 30–35 °C, pH 5.0), producing an ethanol concentration of 4.79 g L− 1 with negligible residual sugar. These results demonstrate the feasibility of one-pot conversion of CRW to bioethanol. The novel enzyme-assisted process is scalable and low-cost, offering a sustainable strategy to valorize slaughterhouse waste into renewable bioethanol. © The Author(s) 2026.
Department of Chemical Engineering, Universitas Negeri Semarang, Semarang, 50229, Indonesia; Research Center of Nanotechnology System, National Research and Innovation Agency (BRIN), Banten, 15314, Indonesia; Research Center for Sustainable Production System and Life Cycle Assessment, National Research and Innovation Agency (BRIN), Banten, 15314, Indonesia; Department of Chemical Engineering, Politeknik ATI Padang, Padang, 25171, Indonesia; Department of Chemical Engineering (BK21 FOUR Graduate Program), Dong-A University, Busan, 49315, South Korea; Department of Chemical Engineering, Universitas Diponegoro, Semarang, 50275, Indonesia; Department of Mechanical Engineering, Universitas Negeri Semarang, Semarang, 50229, Indonesia; Department of Chemistry, Dong-A University, Busan, 49315, South Korea; DAU G-LAMP Project Group, Institute of Natural Science, Dong-A University, Busan, 49315, South Korea; DAU G-LAMP Project Group, Innovation of Center for Atomic Science, Dong-A University, Busan, 49315, South Korea
