Bambang Cahyono, Nur Afiyah, Meiny Suzery, Damar Nurwahyu Bima, Nur Dina Amalina
A high-performance liquid chromatography (HPLC) method was developed for quantifying hesperidin from various orange peel extracts. The development of the HPLC method was carried out to obtain optimal conditions for determining Hesperidin to obtain a single specific chromatogram peak from the standard hesperidin. Identification of various orange peel extracts requires a specific and single chromatogram peak, so that it will easier and obtained more accurate data in determining hesperidin contents. We vary the solvent of hesperidin, eluent composition and flow rate. The solvent variations used were a mixture of water: methanol, a mixture of methanol: DMSO and DMSO. DMSO solvent produces the most homogeneous solution than other solvents. DMSO solvent also produces the least number of chromatogram peaks from the hesperidin standard. The best condition for determining Hesperidin contents, which produces a single chromatogram peak chromatogram, was achieved with the specific condition: the detection wavelength was set at 280 nm and analysis was run at a 35°C with a flow rate 1 mL/min for 10 minutes running time. The eluent composition used as a mobile phase is methanol: acetonitrile: acetic acid (0,1%) (89.3: 10: 0.7), while C-18 column as a stationary phase. The retention time of standard hesperidin was obtained at 2.138 min. With that HPLC condition, the amount of hesperidin content in siam jember (C. Nobilis), bali (C. Maxima), baby java (C. S. Linn Osbeck) and peras (C. Sinensis) oranges peel extract were respectively 6.49%, 22.91%, 8.37%, 7.09%. © 2023 Author(s).
Department of Chemistry, Faculty of Sciences and Mathematics, Diponegoro University, Semarang, Indonesia; Pharmacy Study Program, Faculty of Mathematics and Natural Sciences, Semarang State University, Semarang, Indonesia
