Fidia Fibriana, Apichat Upaichit, Benjamas Cheirsilp
Oily wastes have been widely used to produce lipases, but there is insufficient knowledge on their use to efficiently produce cell-bound lipases (CBLs). This research aimed to optimize yeast–bacterium CBLs production using blended oily wastes by statistical optimization and their potential applications in biodiesel production and wastewater bioremediation. The co-culture of Magnusiomyces spicifer AW2 and Staphylococcus hominis AUP19 produced CBLs as high as 4709 U/L with cell biomass of 23.4 g/L in a two-fold diluted palm oil mill effluent (POME) added by 2.08% (v/v) waste frying oil, 1.72.0% (w/v) ammonium sulfate, 0.1% (w/v) Gum Arabic as an emulsifier (initial pH at 7.0) within 24 h. The CBLs were successfully applied as whole-cell biocatalysts to produce biodiesel through esterification and transesterification with 76% and 87% yields, respectively. Direct application of CBLs for bioremediation of heat-treated various POME concentrations achieved 73.3% oil and grease removal and 73.6% COD removal within 3 days. This study has shown that the blended oily wastes medium was suitable for low-cost production of yeast–bacterium CBLs and their potential applications in solvent-free biodiesel production and wastewater bioremediation. These strategies may greatly contribute to economical green biofuel production and waste biotreatment. © 2022 by the authors.
Molecular Biotechnology Laboratory, Faculty of Agro-Industry, Prince of Songkla University, Hat Yai, 90110, Thailand; Department of Integrated Science, Faculty of Mathematics and Natural Sciences, Universitas Negeri Semarang, Semarang, 50229, Indonesia; Center of Excellence in Innovative Biotechnology for Sustainable Utilization of Bioresources, Faculty of Agro-Industry, Prince of Songkla University, Hat Yai, 90110, Thailand
