Mohamad Endy Yulianto, Ari Yuniastuti, Dadan Rohdiana, Vita Paramita, Rizka Amalia, Sutrisno, Indah Hartati, Eflita Yohana, Shabri, Mega Mustikaningrum, Retno Dwi Nyamiati, Mila Hardiana
Theaflavin (TF) is a component of fermented (enzymatic oxidation) catechin compounds synthesized during tea leaf fermentation, giving a golden yellow color, taste, and aroma to black tea. Theaflavins, which are rich in antioxidants, can treat cancer, obesity, diabetes and are potential as an inhibitor in SARS-CoV-2. Photo fermentation using ultraviolet (UV) has the advantage of penetrating the stoma cell wall in tea leaves and accelerating the oxidation of tea leaves that have gone through the milling process before the fermentation stage. This study aimed to optimize the photo fermentation process of theaflavin compounds from fresh tea leaves through response surface methodology (RSM). The highest theaflavin was obtained at a flow rate of 6 L/minute and a long irradiation time of 55 min, with a theaflavin content of 0.6705%. RSM states that the oxygen flow rate is the most influential factor with an R-Square value of 0.60814 means only 60.814% variation in data can be described by the model. © 2022
Department of Industrial Chemical Engineering Technology, Vocational School of Diponegoro University, Jl. Prof Sudarto No 13 Tembalang, Semarang, 50275, Indonesia; Department of Biology, Semarang State University, Gunungpati, Semarang, 50229, Indonesia; Tea and Kina Research Center, Gambung, Bandung, 40264, Indonesia; Department of Mechanical Engineering, Vocational School of Diponegoro University, Jl. Prof Sudarto No 13 Tembalang, Semarang, 50275, Indonesia; Department of Chemical Engineering, Wahid Hasyim University, Jl Menoreh Tengah X No 22, Semarang, 50232, Indonesia; Department of Mechanical Engineering, Diponegoro University, Jl. Prof Sudarto No 13 Tembalang, Semarang, 50275, Indonesia; Departement of Chemical Engineering, University of muhammadiyah Gresik, Jl. Sumatera No 101, Gresik, 61121, Indonesia; Department of Chemical Engineering Technology, Faculty of Industrial Technology, Pembangunan Nasional “Veteran” Yogyakarta University, Yogyakarta, 55283, Indonesia
