Jendri Mamangkey, Dwi Suryanto, Erman Munir, Apon Zaenal Mustopa, Mada Triandala Sibero, Lucas William Mendes, Adrian Hartanto, Steven Taniwan, Maria Julissa Ek-Ramos, Arman Harahap, Amit Verma, Edy Trihatmoko, Wendry Setiyadi Putranto, Lukas Pardosi, La Ode Adi Parman Rudia
Mangrove-associated bacteria are of industrial interest due to their diverse and versatile enzyme properties. This study investigates the culturable bacteria from a wide range of habitat in a Bruguiera cylindrica mangrove ecosystem in North Sumatra. Screening of extracellular hydrolytic enzymes showed multiple potential traits in amylase, cellulase, chitinase, phosphatase, protease, and urease production by bacterial isolates. Molecular identification based on 16S rDNA region of a potential strain, Vibrio alginolyticus Jme3−20 is then reported as a newly proteolytic agent. The strain also showed a stable growth under salinity (NaCl) stress with considerable phosphate solubilization activities. Protease activity was enhanced by optimizing the 0.5 % (w/v) sucrose and soy peptone in the fermentation medium. SDS-PAGE and zymogram analysis showed the presence of a 35-kDa MW protease. Hence, our study revealed important insights into the bacterial diversity and activity in mangrove ecosystems, evidencing the importance of microbial exploration in this ecosystem. © 2021 The Authors
Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Sumatera Utara, Medan, 20155, Indonesia; Research Center for Biotechnology, Indonesian Institute of Science, Jl. Raya Bogor Km. 46, Cibinong, 16911, West Java, Indonesia; Department of Marine Science, Faculty of Fisheries and Marine Science, Universitas Diponegoro, Jl. Prof. Soedarto S.H., Tembalang, Semarang, 50275, Central Java, Indonesia; Natural Product Laboratory, Integrated Laboratory for Research and Services, Universitas Diponegoro, Jl. Prof. Soedarto S.H., Tembalang, Semarang, 50275, Central Java, Indonesia; Cell and Molecular Biology Laboratory, Center for Nuclear Energy in Agriculture CENA, University of Sao Paulo USP, Piracicaba, Brazil; Department of Agricultural Sciences, University of Helsinki, Helsinki, 00014, Finland; Universidad Autónoma de Nuevo León, Facultad de Ciencias Biológicas, San Nicolás de los Garza, Nuevo León, Mexico; Faculty of Teacher Training and Education, Universitas Labuhanbatu, Rantauprapat, Indonesia; Department of Biochemistry, College of Basic Science and Humanities, SD Agricultural University, 385506, Gujarat, India; Department of Geography, Universitas Negeri Semarang, Semarang, 50229, Indonesia; Faculty of Animal Husbandry, Padjadjaran University, Jatinangor, 45363, Indonesia; Biology Study Program, Faculty of Agriculture, Universitas Timor, Kefamenanu, 85613, Indonesia; Faculty of Mathematics and Natural Sciences, Halu Oleo University, Jalan H.E.A. Mokodompit, Kampus Baru, Kampus Hijau Bumi Tridharma Anduonohu, Kendari, 93232, Indonesia
