W. Christijanti, A.Z. Juniarto, L.B. Suromo
The aim of this study is to determine the correlation between total antioxidant status (TAC) with malondialdehyde (MDA) and caspase 3. This study was divided into non-diabetic (n = 8) and type 2 diabetic rats (n = 8) induced by streptozotocin 65 mg / kg body weight. Epididymal fluid was examined after 28 days. Antioxidant status was examined by the trolox equivalent method, MDA with the thiobarbituric acid (TBA) reaction and caspase 3 expression with immunohistochemistry. Our results show that the mean of TAC in non-diabetic was significantly higher (P <0.000) than diabetic rats, MDA (P <0.000) and caspase 3 expression (P <0.001) in non-diabetic rats were significantly lower than diabetic rats. TAC was negative correlation with MDA (r = 0.881, P = 0.004) and caspase 3 (r = 0.898, P = 0.002) in the non-diabetic group. In the diabetic group, TAC was a negative correlation with MDA (r = 0.856, P = 0.007) and caspase 3 (r = 0.886, P = 0.003). In conclusion, total antioxidants have a negative correlation with MDA and caspase 3 expression. © Published under licence by IOP Publishing Ltd.
Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Semarang, Indonesia; Faculty of Medicine, Universitas Diponegoro, Semarang, Indonesia
