Alia Tsabitha Putricia, Amin Retnoningsih, Kusumadewi Sri Yulita, Syaiful Anwar, Rafdinal, Anton Kamaruddin, Mohamad Reza Tirtawinata
Durian (Durio zibethinus) is a promising tropical fruit and commodity in the domestic and international markets. Indonesia’s durian production has increased over the past two years, but only one-third is suitable for export, as the fruit quality does not yet meet market standards. The regions of Sanggau and Singkawang in West Kalimantan are home to several superior durian varieties that thrive in the forests. Characterizing the genetic diversity of these superior durians is crucial for the provision of high-quality durian seedlings. Genetic diversity characterization using molecular markers is more efficient, effective, and independent of environmental factors. Inter Simple Sequence Repeat (ISSR) is a molecular marker that is relatively simple, fast and does not require prior knowledge of the target genome. This study aims to analyze the genetic diversity of superior durians from Sanggau and Singkawang using ISSR markers. The study detected the allele profiles of 21 superior durian accessions from PCR results using 11 ISSR primers. The results indicated a high polymorphic percentage of 91.73%. Specific alleles were identified in 12 accessions, with a total of 24 specific alleles. This study demonstrates that the genetic diversity of superior durians from Sanggau and Singkawang, as revealed by ISSR markers, is relatively high, because each accession originated from a seed. © 2026 The author(s).
Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Semarang, Central Java, Semarang City, Indonesia; Ecology and Ethnobotany Research Center, Biology and Environment Organization, National Research and Innovation Agency, West Jawa, Bogor Regency, Indonesia; Department of Agroecotechnology, Faculty of Animal and Agricultural Sciences, Universitas Diponegoro, Central Java, Semarang City, Indonesia; Department of Biology, Faculty of Mathematics and Natural Science, Universitas Tanjungpura, West Kalimantan, Kubu Raya Regency, Indonesia; Department of Food Crops and Horticulture of West Kalimantan Province, West Kalimantan, Pontianak City, Indonesia; Indonesian Durian Foundation, Bogor, Indonesia
