From insect waste to clean water: sustainable chitin extraction and its potential application in dye-contaminated wastewater adsorption

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Salwa Salsabela, Fidia Fibriana, Andhina Putri Heriyanti, Amnan Haris, Inaya Sari Melati

2026 Biomass Conversion and Biorefinery Vol. 16 Issue 7 Article Cited by 1 Quartile

Abstract

Troso fabric is an esteemed traditional Indonesian weaving technique incorporating a dyeing process using direct yellow. This dyeing process generates hazardous wastewater, which poses significant health risks due to the carcinogenic and mutagenic properties of the dye upon prolonged exposure. Efficient and environmentally friendly treatment methods are required before discharging wastewater into water bodies. This research aims to extract chitin from black soldier fly (BSF) pupal shells using green chemicals and natural deep eutectic solvent (NADES). Chitin was then characterized and determined for its adsorption capacity on direct yellow-contaminated wastewater from the Troso fabric processing. The determination of the optimum adsorption capacity was carried out using one-factor-at-a-time (OFAT) method. It was found that BSF pupal shell chitin has similar characteristics to commercial shrimp shell chitin, and the adsorption capacity was found to be 21.396 mg/g at 0.1 g chitin mixed with wastewater without stirring in alkaline conditions (pH 11) and 60 min of contact time with adsorption efficiency of 77%. These results were statistically significant (p < 0.05) based on ANOVA analysis. This study shows that chitin from BSF pupal shells effectively adsorbs direct yellow dye, which offers a sustainable solution for hazardous dye-contaminated wastewater treatment. Additionally, the cost of producing BSF pupal shell chitin is significantly lower compared to shrimp shell chitin, making it a more economical option. Further research, optimization, scale-up, environmental impact assessment, and industry collaboration are recommended. © The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2026.

Affiliations

Student of Environmental Science Undergraduate Study Program, Faculty of Mathematics and Natural Sciences, Universitas Negeri Semarang, Central Java, Semarang, 50229, Indonesia; Faculty of Mathematics and Natural Sciences, Universitas Negeri Semarang, Central Java, Semarang, 50229, Indonesia; Faculty of Economics and Business, Universitas Negeri Semarang, Central Java, Semarang, 50229, Indonesia; Faculty of Mathematics and Natural Sciences, Gedung D5 Lantai 1 FMIPA UNNES, Universitas Negeri Semarang, Kampus Sekaran, Kecamatan Gunungpati, Kota Semarang, Central Java, Semarang, 50229, Indonesia