Wulan Christijanti, Achmad Zulfa Juniarto, Lisyani B. Suromo
Background: Sperm DNA fragmentation and the presence of free radicals have effects on the male infertility. Aloe vera peel is assumed to increase antioxidant enzymes. Objectives: The aim of this study was to determine the effect of Aloe vera peel extract on the DNA fragmentation index (DFI) and Malondialdehyde (MDA) levels of diabetic rats. Method: The design of this study was posttest only randomized control group design. Eighteen rats were divided into three groups: the control group (A), group-B and C. Two latter groups were given 100 mg and 200 mg/kg of Aloe vera peel extract for 28 days respectively. Data were obtained from sperm DNA fragmentation and MDA levels from epididymal fluid and serum. Results: Aloe vera peel extract significantly influenced MDA levels between groups (p<0.05). Malondialdehyde epididymal fluid in the control group was higher (9.44±0.39) than group B (3.37±0.38) and group C (3.29±0.31). While MDA serum in the group C (3.07±0.34) was lower than group B (6.06±0.45) and control (9.07 ± 0.45). The mean value of sperm DFI in the control was significantly higher (9.92±3.68) than group B (6.00±1.41) and group C (4.08±1.46), but no significant difference between group B and C (p<0.05). Conclusion: Aloe vera peel extract reduced sperm DFI and MDA levels of epididymal fluid and serum. © 2019 Lahore Medical And Dental College. All rights reserved.
Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Semarang, Semarang, Indonesia; Faculty of Medicine, Universitas Diponegoro, Semarang, Indonesia
