N. Setiati, D. Mustikaningtyas, N.K. Dewi, Partaya
Breeding is main factor to evaluate individual superiority in livestock population. Early estimation is very useful in efficient selection process because it can make short interval generation in seed selection. The aim of this study is to measure breeding value and the influence of GH gene generation to quail in 4 weeks old. 100 female quails an 20 female quails in 4 weeks old are divided in high body weight and low body weight, and collecting the blood sample. The DNA is isolated, amplified and continued with PCR-RFLP. Breeding value are counted based on genotype from Mspl enzyme digestion. GH gene frequency are obtained by AA genotype = (Σ genotype AA / Σ in population) ? 100%, AB = (Σ genotype AB / Σ in population) ? 100%, BB = (Σ genotype BB / Σ in population) ? 100%. The result of this study are obtained for low body weigt AA = 21,15, AB = 20,70 and BB = 20,25, AA = 23,02, AB = 22,38 and BB = 21,75. GH gene frequency is low body weight AA = 0,21, AB = 0,49 and BB = 0,30, while high body weight AA = 0,47, AB = 0,43 and BB = 0,10. © 2019 IOP Publishing Ltd. All rights reserved.
Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Semarang, Indonesia
