Dyah Rini Indriyanti, Suputa, Siti Nur Jannah
Bactrocera is one of important fruit flies. There are many Bactrocera species attack fruits consumed by human or not. We found Bactrocera sp. from forest that attack the fruit that not consumed by human (wild fruit), the species is still doubtful. The morphological identification show that Bactrocera sp has most of the same morphological characters with Bactrocera calumniata and has the same wings character with the B. cucurbitae. The study aimed to confirm the taxonomic status of Bactrocera sp using identify the cytochrome oxidase I gene of mitochondrial DNA and its phylogenic. The methods included fresh larvae DNA isolation, polymerase chain reaction (PCR), electrophoresis, and sequencing. Data analysis using BLASTn program and MEGA software version 6.06 programs. The results showed that sequence (435 bp) of the Bactrocera sp. had highest similarity to B.cucurbitae (100%) (GenBank Acc Number DQ006875.1), and 96% homology with B.calumniata (96%) (GenBank Acc Number GQ154088.1). The Phylogenetic clearly showed that Bactrocera sp have the same common ancestor that came from Switzerland B.cucurbitae. © 2006-2017 Asian Research Publishing Network (ARPN).
Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Semarang, Indonesia; Studies Program of Pests and Plant Diseases, Faculty of Agriculture, Gadjah Mada University, Indonesia
