Prasetyastuti, Atika Rifiani, Gita Larasati, Sri Mursiti, Sri Lestari Sulistyo Rini
Background and Objective: Diabetes mellitus is a group of metabolic diseases with characteristic hyperglycemia. Hyperglycemia leads to excessive free radical production. There is increasing evidence that active compounds of medicinal plants may be used to treat diabetes. This study investigated the effects of 7-hydroxy-2-(4-hydroxy-3-methoxy-phenyl)-chroman-4-one from Swietenia macrophylla King seed on lipid profile, atherogenic index and adipose tissue Peroxisome Proliferator-Activated Receptor gamma (PPAR-γ) expression in type 2 diabetic rats. Methods and Materials: A total of 36 rats with age of eight weeks and weighing an average of 200 grams were used. Rats were divided into 6 groups as follows: A) normal, B) diabetic rats, C) diabetic rats with metformin, D), E) and F) diabetic rats with 7-hydroxy-2-(4-hydroxy-3-methoxy-phenyl)-chroman-4-one (Swietenia macrophylla King) seed doses 10, 30 or 90mg/200g body weight (BW), respectively, were administered treatment orally by gavages. Blood samples were collected for assessment of lipid profile, atherogenic index, before and after 4 weeks administration and at the end of the study, test animals were euthanized. The adipose tissue was used for PPAR-γ expression assessment. Data were analyzed by paired t test and One-Way ANOVA followed by Games-Howell test. Results: Supplementation with three different doses of extract from Swietenia macrophylla King seed in streptozotocin induced diabetic rats for four weeks significantly (p<0.001) reduced cholesterol, triglyceride, low density lipoprotein and increased high density lipoprotein, reduced atherogenic index and increased PPAR-γ expression. Conclusions: These findings demonstrate that supplementation with 7-hydroxy-2-(4-hydroxy-3-methoxy-phenyl)-chroman-4-one from Swietenia macrophylla King seed, improves lipid profile, atherogenic index and increases PPAR-γ gene expression. © 2018, Pharmainfo Publications. All rights reserved.
Department of Biochemistry, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada, Indonesia; Department of Physiology, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada, Indonesia; Medical Faculty, Faculty of Medicine, Public Health and Nursing Universitas Gadjah Mada, Indonesia; Department of Mathematics and Sciences, Universitas Negeri Semarang, Indonesia
