Widya Hary Cahyati, Widya Asmara, Sitti Rahmah Umniyati, Budi Mulyaningsih
Generally, the control of Aedes aegypti mosquito has been achieved using the lethal ovitrap. Later, it is found that the papaya leaves juice inhibits the development of eggs into larvae and also larvae into pupae. This study was conducted to determine the larvicidal effect of papaya leaves juice against the larvae of Aedes aegypti and to analyze the phytochemicals compounds of leaves extract. Phytochemical assays were performed by UV-Vis Spectrophotometry, Thin Layer Chromatography (TLC), and High-Performance Liquid Chromatography (HPLC). The results of the analysis showed that LC50 and LC90 of the papaya leave juice to instar 1 larvae were 4.1% and 15.5%, respectively; whereas the LC50 and LC90 to instar 3 larvae were 10.6% and 18.3%, respectively. Moreover, the results of the phytochemical assays showed that papaya leaves juice have 0.25% alkaloids, 0.14% flavonoid, 0.30% saponin, ≤ 68 mg/L steroid, and 11.34% tannin. In conclusion, papaya leaves extract work with its compounds in serving the biolarvicidal effects against the larvae of Aedes aegypti. © 2019 Journal of International Dental and Medical Research.
Department of Public Health, Faculty of Sports Sciences, Universitas Negeri Semarang, Semarang, 50229, Indonesia; Department of Microbiology, Faculty of Veterinary Medicine, Gadjah Mada University, Yogyakarta, 55281, Indonesia; Department of Parasitology, Faculty of Medical, Gadjah Mada University, Yogyakarta, 55281, Indonesia
