The pathogenicity of entomopathogenic nematodes against Spodopteraexigua

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Dyah Rini Indriyanti, Baharuddin Achmad Fauzi, Yoris Adi Maretta

2017 ARPN Journal of Engineering and Applied Sciences Vol. 12 Issue 24 Article Cited by 0 Quartile

Abstract

Spodopteraexigua is an insect pest that threat to a wide range of agricultural crops. Entomopatho genicnematodes are the parasitic nematodes that have the ability to attack the insects pest. The study to analyzed the pathogenicity of the entomopathogenic nematodes (EPN) from Steinernematidaein controlling the third instar larvae of S. exigua. The study's design was completely randomized design (CRD) with six treatment groups and four times of repetition (10 larvae at each repetition). S. Exigua were taken from the field, andmass breedingwas doneat Laboratoryfor two generation.EPN was isolated from organic soil. EPN breeding carried out using a Tenebriomolitor caterpillar.Third larvae of S. exigua were used for Bioassay test. The number of larvae killed by EPN was recorded for 96 hours.The data analysis used probit analysis from Minitab 1.5 software. The results showed that EPN gave positive response against larvae of S. exigua within 96 hours. The conclusion was LD90 of EPN against the larvae of S. exiguawas 772 IJ/2 ml. © 2006-2017 Asian Research Publishing Network (ARPN).

Affiliations

Biology Department, Faculty of Mathematics and Natural Sciences, Universitas Negeri Semarang, Jalan Raya Sekaran, Gunungpati Semarang, Indonesia