A. Retnoningsih, R. Megia, A. Hartana
In this study, microsatellite markers were used to identify Musa genomic groups and to investigate genetic relationships among banana accessions from various areas in Indonesia. One hundred sixteen banana accessions were analysed using MaCIR108 and Ma-3-90 primer pairs for identifying genomic groups. Six additional SSR primer pairs were used for genetic relationship analysis. The results showed that 73 accessions should be classified in the AA/AAA and AAA genomic groups, two accessions in the BB genomic group, 21 accessions in the AAB genomic group and 20 accessions in the ABB genomic group. Ninety-nine out of the 116 accessions were unique genotypes while the rest were synonyms. The dendrogram generated by UPGMA analysis separated the 116 banana accessions into two main clusters with a similarity of 0.13. All banana accessions belonging to the BB, ABB and AAB genomic groups clustered in the first main cluster, together with the majority of the accessions containing the pure A genome. The second main cluster was formed of 11 accessions of AA/AAA and AAA genome. Within the first main cluster, the accessions containing the B genome were clustered according to their genomic group, except four AAB accessions clustering with accessions containing the A genome alone. The ABB genomic group appeared closer to the BB than to the AAB genomic group. The AA and AAA banana accessions could not be significantly distinguished, although the majority of accessions tended to be clustered according to their ploidy level.
Department of Biology, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University, Indonesia; Department of Biology, Faculty of Mathematics and Natural Sciences, Semarang State University, Indonesia
