Noor Aini Habibah, Sukarti Moeljopawiro, Kumala Dewi, Ari Indrianto
Background and Objective: Stelechocarpus burahol is a plant containing flavonoid compounds that have the potential for use as an antihyperuricemic for gout medication. This study was performed to assess flavonoid production, growth and cell differentiation of S. burahol in cell suspension culture. Methodology: Mesocarp was planted in Murashige and Skoog (MS) medium supplemented with 7.5 mg LG–1picloram for the induction of callus. Non-embryonic callus obtained was used in the formation of cell suspension cultures. Growth of cells was determined by fresh and dry weights. During the culturing, the fresh weight, dry weight and flavonoid content were determined as a result of culture status. Results: The growth of the S. burahol cell suspension was slow, the stationary phase occurred at 30 days. The production of flavonoids was not in line with the growth of cells and the maximum production occurred on the 15th day of the log phase. The globular-shaped cells dominated the cell suspension culture at all ages. Fluorescein diacetate (FDA) staining of cells derived from cell cultures aged for 36 days showed that some cells were still viable. Conclusion: The results show that flavonoid production, growth and cell differentiation of a S. burahol cell suspension culture differed according to the culture age. © 2017 Noor Aini Habibah et al.
Faculty of Biology, Universitas Gadjah Mada, Yogyakarta, 55281, Indonesia; Laboratory of Biochemistry, Faculty of Biology, Universitas Gadjah Mada, Yogyakarta, 55281, Indonesia; Laboratory of Plant Physiology, Faculty of Biology, Universitas Gadjah Mada, Yogyakarta, 55281, Indonesia; Laboratory of Biotechnology, Faculty of Biology, Universitas Gadjah Mada, Yogyakarta, 55281, Indonesia; Laboratory of Plant Tissue Culture, Faculty of Science and Mathematics, Universitas Negeri Semarang, Semarang, Indonesia
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